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Can demyelination lesion volume be accurately determined in the following cervical MRI sequences?

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Hi, I am a neurology intern getting started in multiple sclerosis research and for a beginner like me MRI is quite though matter and still learning a lot. Our centre scans the cervical spine in MS according to the following protocol for their regulair clinical MRI's:
- Sagittal T2 TSE (FoV 200*200, matrix 256x320), slice 3mm and distance 3.3mm
- Sagittal T1 TSE (FoV 200*200, matrix 224x320), slice 3mm and distance 3.3mm
- Sagittal STIR (FoV 200*200, matrix 240x320), slice 3mm and distance 3.3mm
- Axial T2-MEDIC (FoV 180*180, matrix 426x512), slice 3mm and distance 3.3mm

I wondered if these sequences can be used to determine (with reasonable accuracy) MS lesion volume for the cervical spinal cord? Or is the gap (since that slice thickness is 3mm and distance 3.3) a problem for these? Or do the sequences even need to be isotropic in order to determine volumetric information?

Thank you in advance for your insights!

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source https://www.reddit.com/r/Radiology/comments/luyiao/can_demyelination_lesion_volume_be_accurately/
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